The ISMB Biophysics Centre
at Birkbeck

CD Spectroscopy
(Model 202SF, Aviv Biomedical)

Circular dichroism is the property of chiral molecules to absorb the right and left components of circularly polarised light to a different extent. CD spectroscopy measures this differential absorbance.

Nearly all biological macromolecules are chiral and lend themselves to CD experiments. For example the secondary structure content of proteins can be analysed as alpha helices, beta strands and random coil have characteristic spectra. The temperature dependence of the CD signal gives information about protein stability and folding.

Our Aviv CD spectrometer gives excellent quality scans down to 190 nm wavelength enabling quantitative secondary structure analysis. It is equipped with a thermostatted sample chamber for melting experiments.

ISMB Biophysics Centre, Birkbeck, University of London
Last modified 2011